Integrating directed evolution, rational computation, and artificial intelligence technologies, we provide de novo design and directed modification of high-performance enzymes for clients in the industrial catalysis and biopharmaceutical fields. This service overcomes the bottleneck of traditional enzyme modification relying on extensive experimental screening. Based on our self-developed algorithms, we achieve synergistic optimization of sequence, structure, and function, successfully obtaining multiple new sequences with higher activity than natural enzymes in projects already delivered to marketed pharmaceutical companies.